A novel expression vector for transfection of bovine MHC class I genes |
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| Affiliation: | 1. Rajiv Gandhi Cancer Institute and Research Centre, New Delhi, India;2. Christian Medical College, Vellore, Tamil Nadu, India;3. Advanced Medical Research Institute, Bhubaneswar, Odisha, India;4. CORE Diagnostics, Gurgaon, Haryana, India;5. Apollo Hospitals, Bhubaneswar, Odisha, India;1. Department of Psychology & Neuroscience, Temple University, United States of America;2. Neuroscience Program, Temple University, United States of America |
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| Abstract: | A vector is described for the expression of genomic or cDNA copies of bovine major histocompatibility complex (MHC) class I genes in transfected mouse Ltk− cells. Class I gene fragments are amplified by the polymerase chain reaction, using primers in conserved parts of exon 2 and the 3′-untranslated region of the gene. Amplified class I gene fragments can then be subcloned into the expression vector, pBoLA-21, which contains the necessary 5′-and 3′-sequences for correct expression. The vector was tested by subcloning and expressing genomic and cDNA clones. |
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