Plant virus movement protein dynamics probed with a GFP-protein fusion |
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| Affiliation: | 1. Unidad de Investigación, Sociedad Española de Reumatología, Madrid, España;2. Servicio de Reumatología, Hospital Universitario Puerta de Hierro, Majadahonda, Madrid, España;3. Servicio de Reumatología, Hospital de Sant Joan Despí Moisès Broggi, Consorci Sanitari Integral, Sant Joan Despí, Barcelona, España;4. Servicio de Reumatología, Hospital General de Jerez de la Frontera, Jerez de la Frontera, Cádiz, España;5. Servicio de Reumatología, Hospital Universitari i Politècnic La Fe, Valencia, España;6. Servicio de Reumatología, Hospital Universitario Central de Asturias, Oviedo, Asturias, España;7. Servicio de Reumatología, Hospital Universitario Marqués de Valdecilla, Santander, Cantabria, España;8. Servicio de Reumatología, Hospital Universitario Virgen de las Nieves, Granada, España;9. Servicio de Reumatología, Hospital General de Mérida, Mérida, Badajoz, España;10. Servicio de Reumatología, Complejo Hospitalario Universitario A Coruña, A Coruña, España;11. Servicio de Reumatología, Hospital Universitario Virgen de la Macarena, Sevilla, España;12. Servicio de Reumatología, Hospital Universitario Virgen de la Arrixaca, Murcia, España;13. Servicio de Reumatología, Fundación Hospital Alcorcón, Alcorcón, Madrid, España;14. Servicio de Reumatología, Hospital Clínico Universitario de Valladolid, Valladolid, España;15. Servicio de Reumatología, Hospital Universitario de Gran Canaria Dr. Negrín, Las Palmas de Gran Canaria, Las Palmas, España;p. Servicio de Reumatología, Hospital Universitario La Princesa, Madrid, España;q. Servicio de Reumatología, Hospital San Jorge, Huesca, España;r. Servicio de Reumatología, Complejo Hospitalario Universitario de Albacete, Albacete, España;s. Servicio de Reumatología, Hospital Clínic de Barcelona, Barcelona, España;t. Servicio de Reumatología, Hospital General Universitario de Alicante, Alicante, España;1. Clinical Associate (Internal Medicine), University of Illinois College of Medicine at Peoria, Peoria, IL, USA;2. Clinical Gastroenterologist (Gastroenterology and Hepatology), University of Illinois College of Medicine at Peoria, Peoria, IL, USA;3. Clinical Gastroenterologist (Gastroenterology and Hepatology), University of Missouri, Columbia, MI, USA;1. Laboratories of Molecular and Cellular Biology, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwaicho, Fuchu, Tokyo 183-8509, Japan;2. Laboratories of Plant Pathology, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwaicho, Fuchu, Tokyo 183-8509, Japan;1. Guangdong Engineering & Technology Research Centre of Graphene-like Materials and Products, Department of Chemistry, College of Chemistry and Materials Science, Jinan University, Guangzhou, 510632, China;2. Guangdong Engineering & Technology Research Centre of Efficient Green Energy and Environment Protection Materials, Guangdong Provincial Key Laboratory of Quantum Engineering and Quantum Materials, School of Physics and Telecommunication Engineering, South China Normal University, Guangzhou, 510006, China;1. USDA-ARS, Arid-Land Agricultural Research Center, Maricopa, AZ 85138, USA;2. University of Arizona, Yuma Agricultural Research Center, Yuma, AZ, USA |
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| Abstract: | A genetic fusion between the gene encoding green fluorescent protein (GFP) from the jellyfish Aequorea victoria, with that of the Ob-tobamovirus movement protein (MP) resulted in the expression of a fluorescent fusion protein (MP: :GFP) that was fully biologically active in mediating the cell-to-cell spread of the Ob-virus. The MP::GFP fusion was used to follow in planta the subcellular trafficking of MP. GFP-tagged MP was transiently expressed and found to be associated with several subcellular compartments and structures including trans-wall structures, presumably plasmodesmata, and filament structures. The MP::GFP fusion can be used to monitor MP association with host proteins and structures, and for the isolation of interacting host components. |
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