Drosophila melanogaster P1 genomic clone DS05563 contains the chaperonin-encoding gene Cctg |
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| Institution: | 1. College of Horticulture, Shenyang Agricultural University, Shenyang, Liaoning 110866, PR China;2. Key Laboratory of Protected Horticulture of Education Ministry, Liaoning, PR China;3. National and Local Joint Engineering Research Centre of Northern Horticultural, Facilities Design and Application Technology (Liaoning), Shenyang, Liaoning 110866, PR China;1. State Key Laboratory of Multiphase Complex Systems, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China;2. University of Chinese Academy of Sciences, Beijing 100049, China;3. State Key Laboratory of Complex Nonferrous Metal Resources Clean Utilization, Kunming 650093, Yunnan, China;1. Key Laboratory of Urban Agriculture (South) Ministry of Agriculture, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China;2. Department of Ornamental Plants and Horticulture, College of Landscape Architecture, Northeast Forestry University, Harbin 150040, China |
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| Abstract: | We report the sequence analysis of a Drosophila melanogaster (Dm) P1 genomic clone (DS05563) which contains the γ-chaperonin-encoding gene, Cctg. The (Hs) Cctg orthologue was found to share strong sequence identity with a 1603-bp region of DS05563, suggesting that Dm Cctg is located within this region. Detailed analysis has shown that Dm Cctg comprises four exons and is interrupted by three introns of 55, 85 and 66 bp. Dm Cctg encodes a predicted peptide of 545 amino acids (aa) (approx. 60 kDa). The predicted Dm CCTγ aa sequence shares a high degree of sequence identity with γ-orthologues from human (70%), mouse (70%), protozoa (60%) and yeast (60%), and also contains domains found in other chaperonins including bacterial GroEL, mitochondrial Hsp60 and plant Rubisco large subunit-binding protein. These data support the conclusion that the DS05563 clone contains the Dm Cctg gene. |
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