Cloning of the Mycoplasma capricolum gene encoding peptide-chain release factor |
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| Institution: | 1. Department of Biology, School of Science, Nagoya University, Nagoya 464-01, Japan;2. Biohistory Research Hall, 1-1 Murasaki-Cho, Takatsuki, Osaka 569, Japan Tel. (81-726) 81-9752;1. Chair of Bioanalytics, Institute of Biotechnology, Technische Universität Berlin, 13355 Berlin, Germany;2. Wellcome Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3BF, United Kingdom;1. Global Health Institute, School of Life Sciences, École Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland;2. Institute of Bioengineering, School of Life Sciences, École Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland;1. Indian Institute of Science Education and Research, Dr. Homi Bhabha Road, Pashan, Pune 411008, India;2. INRAE, University of Bordeaux, UMR 1332 BFP, Villenave d’Ornon, Bordeaux, France;3. From the Department of Physiology & Biophysics, University of California, Irvine, Irvine, California 92697;;4. Department of Bioengineering and Therapeutic Sciences, Department of Pharmaceutical Chemistry, California Institute for Quantitative Biosciences, University of California, San Francisco, San Francisco, California 94143;;5. Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, Martinsried 82152, Germany;;6. Department of Pharmacology, University of California, San Diego, La Jolla, California, 92093;;12. Department of Biology, Institute of Molecular Systems Biology, Eidgenössische Technische Hochschule (ETH) Zürich, Zurich, Switzerland;;8. Functional Genomics Center Zurich (FGCZ), University of Zurich, ETH Zurich, CH-8057 Zurich, Switzerland;;9. Institute of Molecular Life Sciences, University of Zurich, CH-8057 Zurich, Switzerland;;2. Faculty of Science, University of Zurich, Zurich, Switzerland;1. Graduate School of Science, Osaka City University, Sumiyoshi-ku, Osaka, 558-8585, Japan;2. The OCU Advanced Research Institute for Natural Science and Technology (OCARINA), Osaka City University, Sumiyoshi-ku, Osaka, 558-8585, Japan |
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| Abstract: | In Mycoplasma capricolum (Mc), a relative of Gram+ eubacteria with a high genomic A+T-content, the UGA codon is assigned to Trp instead of being a stop codon. We previously showed the lack of peptide-chain release factor (RF) activity in vitro responding to the UGA codon in this bacterium Inagaki et al., Nucleic Acids Res. 21 (1993) 1335–1338]. To obtain more information on the translation termination mechanism of Mc, we isolated and sequenced the gene encoding RF. The deduced amino-acid sequence has no RF-2-specific + 1 frameshift site and shows 50 and 36% identity to Escherichia coli RF-1 and RF-2, respectively. We conclude that this gene encodes the putative RF-1 which would possess the conserved ‘five-domain’ structure of RF family found in various organisms |
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