Cloning and characterisation of a gene from Plasmodium vivax and P. knowlesi: homology with valine-tRNA synthetase |
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| Affiliation: | 1. Unité d''Immunoparasitologie, CNRS URA1960, Institut Pasteur, 25-28 Rue du Dr. Roux, 75724 Paris, cedex 15, France. Tel. (33-1) 45688000;2. Unité de Parasitologie Experimentale, CNRS URA1960, Institut Pasteur, 25-28 Rue du Dr. Roux, 75724 Paris, cedex 15, France. Tel. (33-1) 45688000;3. Unité Genetique Moléculaire des Levures, Institut Pasteur, 25-28 Rue du Dr. Roux, 75724 Paris, cedex 15, France. Tel. (33-1) 45688000;4. Unité d''Immunologie Structurale, Institut Pasteur, 25-28 Rue du Dr. Roux, 75724 Paris, cedex 15, France. Tel. (33-1) 45688000;5. Malaria Research Unit, Department of Parasitology, Faculty of Medicine, University of Colombo, Colombo 8, Sri Lanka. Tel. (94-1) 699284;1. CHRU Lille, Institut Cœur-Poumon - Bd du Professeur Jules Leclercq - CHU Lille, F59000-Lille, France;2. CHU Brabois, Vandœuvre-lès-Nancy, France;3. Universitaire Lille, CHU Lille, ULR 2694-METRICS: évaluation des technologies de santé et des pratiques médicales, Lille, France;4. L''Institut du Thorax, Cardiologic Department and Reference Center for Hereditary Arrhythmic Diseases INSERM 1087, Nantes, France;1. Center for Clinical Research, Department of Hepatology, Croix-Rousse Hospital, Hospices Civils de Lyon, Lyon, France;2. Department of Infectious Diseases, Centre Hospitalier Universitaire de Nice, Hôpital l''Archet, Nice, France;3. Immuno-hematology Clinic, Assistance Publique – Hôpitaux de Marseille, Hôpital Sainte-Marguerite, Marseille, France;4. Aix-Marseille University, Inserm U912 (SESSTIM), Marseille, France;5. Department of Infectious Diseases, Assistance Publique - Hôpitaux de Paris, Pitié-Salpêtrière Hospital, Paris, France;6. Sorbonne Universités, UPMC Université Paris 06, INSERM, Institut Pierre Louis d''épidémiologie et de Santé Publique (IPLESP UMRS 1136), Paris, France;7. CHU Toulouse, COREVIH, Toulouse, France;8. Université de Toulouse III, Toulouse, France;9. INSERM, UMR, 1027, Toulouse, France;10. Department of Infectious Diseases, UMI 233 INSERM U1175, CHU de Montpellier, Montpellier, France;11. Department of Infectious Diseases, Hotel Dieu Hospital, Nantes, France;12. Department of Infectious Diseases and Travel Diseases, Centre Hospitalier Gustave-Dron, Tourcoing, France;13. Department of Internal Medicine, Infectious Diseases and Clinical Immunology, Hôpital Robert Debré, CHU, Reims, France;14. Université de Reims Champagne-Ardenne, Faculté de médecine, EA-4684/SFR CAP-SANTE, Reims, France;15. HIV Infection Care Centre, Hôpitaux Universitaires, Strasbourg, France;p. Department of Infectious Diseases, CHU, Saint-Etienne, France;q. Department of Infectious Diseases, CHU Clermont-Ferrand, Clermont-Ferrand, France;r. Department of Infectious Diseases, Centre d''Infectiologie Necker-Pasteur, IHU Imagine, Assistance Publique - Hôpitaux de Paris, Hôpital Necker-Enfants Malades, Paris, France;s. Université Paris Descartes, Sorbonne Paris Cité, EA7327, Paris, France;t. Department of Internal Medicine, CHU, Bicètre, France;u. Department of Infectious Diseases, CHRU de Besançon, Besançon, France;v. Faculté de Médecine Hyacinthe Bastaraud, Université des Antilles, and Service de Maladies Infectieuses et Tropicales, Dermatologie et Médecine Interne, and Inserm CIC 1424, Centre Hospitalier Universitaire de Pointe-à-Pitre, Pointe-à-Pitre, France;w. Department of Infectious Diseases, CHU de Martinique, Fort-de-France, France;x. Université des Antilles EA4537 and INSERM CIC1424, Fort-de-France, France;y. Department of Infectious Diseases, Croix-Rousse Hospital, Hospices Civils de Lyon, Lyon, France;z. INSERM U1052, Lyon, France;1. Department of Epidemiology, Harvard TH Chan School of Public Health, Boston, MA, USA;2. Department of Biostatistics, Harvard TH Chan School of Public Health, Boston, MA, USA;3. University College London, London, UK;4. Sorbonne Universités, UPMC Univ Paris 06, INSERM, Institut Pierre Louis d''épidémiologie et de Santé Publique (IPLESP UMRS 1136), Paris, France;5. Assistance Publique-Hopitaux de Paris (AP-HP), Hopital Antoine Béclère, Service de Médecine Interne, Clamart, France;6. Department of Medicine, University of Alabama at Birmingham, Birmingham, AL, USA;7. UAB Center for AIDS Research, University of Alabama at Birmingham, Birmingham, AL, USA;8. Université Paris Sud, INSERM CESP U1018, Paris, France;9. AP-HP, Hopital de Bicêtre, Service de Santé Publique, le Kremlin Bicêtre, France;10. School of Medicine, Division of Allergy and Infectious Diseases, University of Washington, Seattle, WA, USA;11. Department of Medicine, University of Washington, Seattle, WA, USA;12. Institut de Santé Publique, d''Epidémiologie et de Développement, Université de Bordeaux, Bordeaux, France;13. INSERM U897, Centre INSERM Epidémiologie et Biostatistique, Université de Bordeaux, Bordeaux, France;14. Department of Internal Medicine, Centre Hospitalier Universitaire de Bordeaux, Bordeaux, France;15. School of Medicine, Johns Hopkins University, Baltimore, MD, USA;p. Internal Medicine, Johns Hopkins University, Baltimore, MD, USA;q. Stichting HIV Monitoring, Amsterdam, Netherlands;r. Academic Medical Center, Department of Global Health and Division of Infectious Diseases, University of Amsterdam, Amsterdam, Netherlands;s. Amsterdam Institute for Global Health and Development, Amsterdam, Netherlands;t. National Centre of Epidemiology, Instituto de Salud Carlos III, Madrid, Spain;u. Consorcio de Investigación Biomédica de Epidemiología y Salud Pública (CIBERESP), Madrid, Spain;v. Ramón y Cajal Hospital, IRYCIS, Madrid, Spain;w. University of Alcalá de Henares, Madrid, Spain;x. Positive Health Program, San Francisco General Hospital, San Francisco, CA, USA;y. Servei de Medicina Interna, Hospital Universitari Germans Trias i Pujol, Universitat Autònoma de Barcelona, Barcelona, Spain;z. Fenway Health, Boston, MA, USA;11. Hospital Universitari de Bellvitge-Bellvitge Institute for Biomedical Research, Hospitalet de Llobregat, Barcelona, Spain;12. Division of Infectious Diseases, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA;13. Yale School of Medicine, New Haven, CT, USA;14. VA Connecticut Healthcare System, West Haven, CT, USA;15. Southern Alberta HIV Clinic, University of Calgary, Calgary, AB, Canada;16. Programa de Computação Científica, FIOCRUZ, Rio de Janeiro, Brazil;17. Basel Institute for Clinical Epidemiology and Biostatistics, University Hospital Basel, Basel, Switzerland;18. Centre for Infectious Disease Epidemiology and Research, School of Public Health and Family Medicine, Faculty of Health Sciences, University of Cape Town, Cape Town, South Africa;19. University of Bern, Institute for Social and Preventive Medicine, Bern, Switzerland;110. Department of Infectious Diseases, Bern University Hospital and University of Bern, Bern, Switzerland;111. Department of Hygiene, Epidemiology and Medical Statistics, Athens University Medical School, Athens, Greece;112. Infectious Diseases, Hospital Clinic-IDIBAPS, Barcelona, Spain;113. School of Social and Community Medicine, University of Bristol, Bristol, UK;114. Harvard–MIT Division of Health Sciences and Technology, Boston, MA, USA |
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| Abstract: | We have previously described λgt11 clone detected by immune screening with a monoclonal antibody (mAb) A12. This mAb is capable of completely blocking Plasmodium vivax transmission in the mosquito vector. An epitope recognised by A12 was mapped to six amino acids (aa) within the translated sequence of this clone. Here, we describe the complete sequence of the gene within which we mapped this epitope. Surprisingly, the translated sequence of the full-length open reading frame shows homology with that of valine-t-RNA synthetases (Val-tRS) from other organisms. DNA crosshybridisation with several of these species was observed by Southern blot. In addition, the corresponding gene has been obtained from the closely related simian malaria parasite, P. knowlesi. The two as sequences show 66% identity and yet are very divergent from other Val-tRS sequences, apart from conserved blocks related to functional activity. Multiple sequence alignments reflect this dichotomy, as do predicted differences in antigenicity. |
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