Cloning and analysis of a cDNA encoding farnesyl diphosphate synthase from Artemisia annua |
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| Institution: | 1. Department of Food Safety and Infection Biology, Section for Food Safety, Faculty of Veterinary Medicine and Biosciences, Norwegian University of Life Sciences, P.O. Box 8146 Dep., N-0033 Oslo, Norway;2. Department of Community Medicine, Faculty of Health Sciences, UiT The Arctic University of Norway, N-9307 Tromsø, Norway;3. Senja Lab, Sjøgata 5, N-9300 Finnsnes, Norway;1. Air Pollution Laboratory (APL), Centre of Excellence in Environmental Studies (CEES), King Abdulaziz University, P. O. Box 80216, Jeddah 21589, Saudi Arabia;2. Department of Botany, Faculty of Science, Alexandria University, 21526 El Shatby, Alexandria, Egypt |
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| Abstract: | A cDNA encoding farnesyl diphosphate (FPP) synthase (FPPS) has been cloned from a cDNA library of Artemisia annua. The sequence analysis showed that the cDNA encoded a protein of 343 amino acid (aa) residues with a calculated molecular weight of 39 420 kDa. The deduced aa sequence of the cDNA was highly similar to FPPS from other plants, yeast and mammals, and contained the two conserved domains found in polyprenyl synthases including FPPS, geranylgeranyl diphosphate synthases and hexaprenyl diphosphate synthases. The expression of the cDNA in Escherichia coli showed enzyme activity for FPPS in vitro. |
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