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New shuttle vectors for Actinobacillus actinomycetemcomitans and Escherichia coli
Affiliation:1. College of Marine Science and Biological Engineering, Qingdao University of Science & Technology, Qingdao, Shandong 266042, China;2. School of Polymer Science and Engineering, Qingdao University of Science & Technology, Qingdao, Shandong 266042, China;3. Shandong Centre of Crop Germplasm Resources, Jinan, Shandong 250100, China;1. Lancaster Environment Centre, Lancaster University, Lancaster LA1 4YQ, UK;2. Department of Earth, Environmental and Planetary Sciences, Brown University, Providence, RI 02912 USA
Abstract:A series of shuttle vectors for Actinobacillus actinomycetemcomitans (Aa) and Escherichia coli (Ec) was developed. These vectors carry a multiple cloning site, the lacZα reporter gene, the replication origin for Aa derived from pVT736-1 and a replicon for Ec originated from pUC, P15A or pSC101. With these vectors, cloning and expression can be effectively performed in Aa and Ec.
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