Construction of an alkaline phosphatase fusion-generating transposon,mTn10phoA |
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| Affiliation: | 1. Department of Mechanics, Huazhong University of Science and Technology, Wuhan 430074, China;2. Hubei Key Laboratory for Engineering Structural Analysis and Safety Assessment, Wuhan 430074, China;3. Department of Mechanical Engineering, Technische Universität Darmstadt, Darmstadt 64293, Germany;2. Laboratory on the Mechanism and Regulation of Protein Synthesis Eunice Kennedy Shriver National Institute of Child Health and Human Development National Institutes of Health Bethesda, MD, USA |
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| Abstract: | We constructed a derivative of the mini-transposon mTn10 that generates translational fusions to the phoA gene from Escherichia coli and carries the KmR determinant from Tn5. This new transposon, mTn10phoA, is carried on a mobilizable plasmid with both selectable and counterselectable markers. The plasmid carrying mTn10phoA was introduced into Legionella pneumophila. Southern hybridization analysis indicated that the mTn10phoA insertions were randomly distributed |
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