Cell-cycle-dependent expression of the STK-1 gene encoding a novel murine putative protein kinase |
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Affiliation: | 1. Dept. of Orthopedic Surgery, Mayo Clinic, Rochester, MN, USA;2. Dept. of Oncology, Mayo Clinic, Rochester, MN, USA;3. Program of Cellular and Molecular Biology, Institute of Biomedical Sciences (ICBM), Faculty of Medicine, University of Chile, Santiago 8380453, Chile |
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Abstract: | We have cloned a novel putative serine/threonine kinase-encoding gene, designated STK-1, from murine embryonic stem (ES) cell and testis cDNA libraries. The kinase most closely related to STK-1 is Xenopus laevis XLP46 protein kinase which shows 71% amino-acid identity to STK-1 between their kinase domains. Nevertheless, STK-1 is conserved throughout phylogeny with hybridizing sequences being detected in DNA from mammals, amphibians, insects and yeast. STK-1 mRNA is detected in testis, intestine and spleen, tissues that contain a large number of proliferating cells, but not in other tissues. All cell lines tested expressed STK-1 mRNA with levels being dependent upon proliferation rates. In NIH 3T3 cells, STK-1 is expressed in a cell-cycle-dependent fashion. These findings suggest a role for STK-1 in cell growth |
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