Cloning and characterization of the Saccharomyces cerevisiae C-22 sterol desaturase gene,encoding a second cytochrome P-450 involved in ergosterol biosynthesis |
| |
| Affiliation: | 1. Department of Pathology, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, Taiwan, 100;2. Division of Hematology, Department of Medicine, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, Taiwan, 100;3. Graduate Institute of Pathology, National Taiwan University College of Medicine, Taipei, Taiwan, 100;2. Center for Molecular Medicine and Genetics, Wayne State University, 110, East Warren Avenue, Detroit, MI 48201;3. Karmanos Cancer Institute, Wayne State University, 110, East Warren Avenue, Detroit, MI 48201;4. Department of Oncology, Wayne State University, 110, East Warren Avenue, Detroit, MI 48201;5. Department of Pathology, Wayne State University, 110, East Warren Avenue, Detroit, MI 48201;1. Faculty of Pharmaceutical Sciences, Suzuka University of Medical Science, Japan;2. Department of Chemotherapy and Mycoses, National Institute of Infectious Diseases, Japan;3. Division of Pathology, Immunology and Microbiology, Graduate School of Medicine, The University of Tokyo, Japan;4. Department of Electronic and Information Engineering, Suzuka National College of Technology, Japan;5. Department of Biology, Indiana University–Purdue University Indianapolis, USA |
| |
| Abstract: | The ERG5 gene from Saccharomyces cerevisiae was cloned by complementation of an erg5-1 mutation using a negative selection protocol involving screening for nystatin-sensitive transformants. ERG5 is the putative gene encoding the C-22 sterol desaturase required in ergosterol biosynthesis. The functional gene was localized to a 2.15-kb SacI-EcoRI DNA fragment containing an open reading frame of 538 amino acids (aa). ERG5 contains a 10-aa motif consistent with its role as a cytochrome P-450 (CyP450) enzyme and is similar to a number of mammalian CyP450 enzymes. Gene disruption demonstrates that ERG5 is not essential for cell viability |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
