Cloning and characterization of cDNAs coding for heavy and light chains of a monoclonal antibody (MabA34) specific for human plasma apolipoprotein A-I |
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| Institution: | 1. Neurovascular Research Laboratory, Vall d''Hebron Institut de Recerca (VHIR), Universitat Autònoma de Barcelona, Barcelona, Spain;2. Stroke Unit, Hospital Universitari Vall d''Hebron, Barcelona, Spain;3. Neurology Department, Biomedicine Institute of Seville (IBIS), University Hospital Virgen del Rocio, Seville, Spain |
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| Abstract: | We have determined the nucleotide (nt) sequences encoding the heavy (H)- and light (L)-chains of the Fab fragment of a murine monoclonal antibody, MabA34 (γ,κ), which is specific for human plasma apolipoprotein A-I of high-density lipoproteins. The variable (V) regions of the H- and L-chains were revealed to be members of mouse H-chain subgroup II(A) and κ L-chain subgroup II, respectively. A few unusual amino acids in the V region of the H-chain, and nt residues probably introduced by somatic mutations from germline genes were also identified. |
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