A versatile plasmid expression vector for the production of biotinylated proteins by site-specific,enzymatic modification in Escherichia coli |
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| Institution: | 1. Kaiser Permanente, TPMG Northern California Regional Laboratory, Berkeley, CA, United States;2. University of Washington, Department of Laboratory Medicine and Pathology, Seattle, WA, United States;3. University of British Columbia, Department of Pathology and Laboratory Medicine, Vancouver, BC, Canada;1. Kaiser Permanente, TPMG Northern California Regional Laboratory, Berkeley, CA, USA;2. University of British Columbia, Department of Pathology and Laboratory Medicine, Vancouver, BC, Canada |
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| Abstract: | A versatile plasmid vector was designed to direct the synthesis of recombinant proteins in either one of two forms that will be biotinylated in Escherichia coli with high efficiency at a single, unique site. The protein of interest can be produced with a peptide substrate for E. coli biotin holoenzyme synthetase (BirA) joined directly to its N terminus, or alternatively, as a fusion to the C terminus of a maltose-binding protein domain (Ma1E) with the peptide substrate on its N terminus. To maximize the yield of biotinylated protein, the vector is designed to express the substrate in a coupled translation arrangement with the enzyme |
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