Controlled Gating of Lysenin Pores |
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Authors: | Daniel Fologea Eric Krueger Rachel Lee Matt Naglak Yuriy Mazur Ralph Henry Greg Salamo |
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Affiliation: | 1. Department of Biological Sciences, University of Arkansas, Fayetteville, AR72701, USA;2. Department of Physics, University of Arkansas, Fayetteville, AR72701, USA |
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Abstract: | Lysenin forms unitary large conductance pores in artificial bilayer membranes containing sphingomyelin. A population of lysenin pores inserted into such a bilayer membrane exhibited a dynamic negative conductance region, as predicted by a simple two-state model for voltage-gated channels. The recorded I-V curves demonstrated that lysenin pores inserted into the bilayer are uniformly oriented. Additionally, the transition between the two-states was affected by changes in the monovalent ion concentration and pH, pointing towards an electrostatic interaction governing the gating mechanism. |
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Keywords: | Lysenin Voltage gated pores Negative conductance |
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