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Structure and conformational studies on dityrosine formation in the DNA binding domain of RFX5
Authors:Madhumita Chakraborty  Dipankar Bhattacharya  Chaitali Mukhopadhyay  Abhijit Chakrabarti
Affiliation:1. Biophysics Division and Structural Genomics Section, Saha Institute of Nuclear Physics, 1/AF, Bidhannagar, Kolkata 700064, India;2. Department of Chemistry, University of Calcutta, Kolkata 700009, India
Abstract:
Keywords:MHC, Major histocompatibility complex   RFX, Regulatory factor X   RFX5DBD, N-terminal DNA binding domain of RFX5   DT, Dityrosine   DTT, Dithiothrietol   HRP, Horseradish peroxidase   UV, Electromagnetic radiation in the ultraviolet region   λmax, Wavelength of maximum fluorescence emission   SDS-PAGE, SDS polyacrylamide gel electrophoresis   SEM, Standard error of the mean   IPTG, Isopropyl β-d-1 thiogalactopyranoside   PMSF, Phenyl methyl sulfonyl fluoride   GST, Glutathione S-transferase   CHCA, α-cyano-4-hydroxycinnamic acid
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