蛋白质双向电泳图像分析

随着人类基因组计划的接近完成，蛋白质组（proteome）研究成为新的热点.其中高分辨率的双向电泳（two-dimensional gel electrophoresis, 2-DE）技术使对组织或细胞的整个蛋白质组的综合分析成为可能.近年来这一技术有了很大的改进和提高，特别是图像分析系统，算法更为先进，功能日益强大，操作也更简便，为大规模研究提供了良好的工具.使用新一代的2D图像分析系统，对离体培养的雪旺氏细胞的蛋白质样品双向电泳结果进行了初步分析，探讨了在图像扫描、点检测、背景消除、匹配、结果报告和数据分析各步中的技术问题，并报告了进行2D图像分析的体会.

The Image Analysis of Two-Dimensional Gel Electrophoresis

Proteome research has become a new hot spot in the post-genome era. High-resolution two-dimensional gel electrophoresis (2-DE), which provides the most comprehensive analysis system of the whole proteome, was highly improved in recent years. With the development of computer techniques, the powerful and user-friendly image analysis systems appeared to help high-throughput, large-scale proteomic studies. Using new generation two-dimensional image analysis software, ImageMaster 2D Elite, the 2D gels of proteins extracted from cultured Schwann’s cells were processed. The analysis procedure, including image acquirement, spot detection, match, background subtraction, pI/Mr calibration, analysis results report and database query, were reported and discussed.