Improvement in thermal stability of l-fucose dehydrogenase by chemical modification and additives for use as an NADPH recycling reagent |
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Authors: | Mikio Bakke Keisuke Furukawa Naoki Kajiyama |
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Institution: | (1) Kikkoman Corporation, 399 Noda, Noda City, Chiba, 278-0037, Japan |
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Abstract: | Chemical modification and addition of stabilizers were evaluated to stabilize l-fucose dehydrogenase (FDH) in mild alkaline which is required for NADPH recycling used in a glutamate dehydrogenase (GDH)-catalyzed elimination of ammonia. FDH lost all its activity at pH 9 after 10 min at 50 °C, while FDH conjugated with dextran retained about 30% activity. EDTA or citric acid, at 50–100 mM, increased the thermal stability of FDH with retention of more than 70–80% activity after heat-treatment. FDH was then functional for NADPH regeneration. |
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Keywords: | chelating reagents chemical modification fucose dehydrogenase NADPH recycling stabilization |
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