MicroRNA‐328 is involved in the effect of selenium on hydrogen peroxide‐induced injury in H9c2 cells |
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Authors: | Xiaolin Zheng Xiaoyan Hu Tangdong Ge Mengdi Li Minxia Shi Jincheng Luo Hehuan Lai Tingting Nie Fenglan Li Hui Li |
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Institution: | Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, Heilongjiang, People's Republic of China |
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Abstract: | Oxidative stress induces apoptosis in cardiac cells, and antioxidants attenuate the injury. MicroRNAs (miRNAs) are also involved in cell death; therefore, this study aimed to investigate the role of miRNAs in the effect of selenium on oxidative stress‐induced apoptosis. The effects of sodium selenite were analyzed via cell viability, superoxide dismutase (SOD) activity, and malondialdehyde (MDA) concentration. Flow cytometry was used to evaluate cell apoptosis. Fura‐2AM was used to calculate intracellular Ca2+ concentration. Sodium selenite could ameliorate hydrogen peroxide (H2O2)‐induced cell apoptosis and improve expression levels of glutathione peroxidase and thioredoxin reductase. Pretreatment with sodium selenite improved SOD activity and reduced MDA concentration. Treatments with H2O2 or sodium selenite decreased miR‐328 levels. MiR‐328 overexpression enhanced cell apoptosis, reduced ATP2A2 levels, and increased intracellular Ca2+ concentration, while inhibition produced opposite effects. MiR‐328 might be involved in the effect of sodium selenite on H2O2‐induced cell death in H9c2 cells. |
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Keywords: | apoptosis microRNA‐328 oxidative stress selenium |
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