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Highly sensitive SERS analysis of the cyclic Arg–Gly–Asp peptide ligands of cells using nanogap antennas |
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| Authors: | Alejandro Portela Taka‐aki Yano Christian Santschi Olivier J F Martin Hitoshi Tabata Masahiko Hara |
| Institution: | 1. Department of Chemical Science and Engineering, Tokyo Institute of Technology, Midori‐ku, Yokohama, Kanagawa, Japan;2. Nanophotonics and Metrology Laboratory, Swiss Federal Institute of Technology Lausanne, Lausanne, Switzerland;3. Bioengineering Department, Engineering School, The University of Tokyo, Bunkyo‐ku, Tokyo, Japan |
| Abstract: | The cyclic RGD (cRGD) peptide ligands of cells have become widely used for treating several cancers. We report a highly sensitive analysis of c(RGDfC) using surface enhanced Raman spectroscopy (SERS) using single dimer nanogap antennas in aqueous environment. Good agreement between characteristic peaks of the SERS and the Raman spectra of bulk c(RGDfC) with its peptide's constituents were observed. The exhibited blinking of the SERS spectra and synchronization of intensity fluctuations, suggest that the SERS spectra acquired from single dimer nanogap antennas was dominated by the spectrum of single to a few molecules. SERS spectra of c(RGDfC) could be used to detect at the nanoscale, the cells' transmembrane proteins binding to its ligand.  SERS of cyclic RGD on nanogap antenna. |
| Keywords: | Raman SERS fluctuations cyclic RGD nanogap antennas plasmonic biophotonics |