1. Fujian Provincial Key Laboratory for Photonics Technology & Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou, China;2. Institute of Bioengineering and Nanotechnology, The Nanos, Singapore, Singapore;3. Singapore‐MIT Alliance for Research and Technology, Singapore, Singapore;4. Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, USA;5. Department of Physiology, Yong Loo Lin School of Medicine, Singapore, Singapore;6. Mechanobiology Institute, National University of Singapore, T‐Lab, Singapore, Singapore
Abstract:
Nanoscopy enables breaking down the light diffraction limit and reveals the nanostructures of objects being studied using light. In 2014, three scientists pioneered the development of nanoscopy and won the Nobel Prize in Chemistry. This recognized the achievement of the past twenty years in the field of nanoscopy. However, fluorescent probes used in the field of nanoscopy are still numbered. Here, we review the currently available four categories of probes and existing methods to improve the performance of probes.
