A Kinetic Examination of Penicillin Acylase Stability in Water-organic Solvent Systems at Different Temperatures |
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| Authors: | Miguel Arroyo Raquel Torres-Guzm n Isabel de la Mata MarÍ a Pilar Castill n Carmen Acebal |
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| Institution: | Miguel Arroyo ,Raquel Torres-Guzmá,n,Isabel de la Mata,MarÍ,a Pilar Castilló,n,Carmen Acebal |
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| Abstract: | We have studied the thermal stability of penicillin acylase from Streptomyces lavendulae in water-organic solvent monophasic systems at the range of temperatures between 40 and 60°C. We found a linear correlation between the log λP value of the solvent and the activation free energy for denaturation ( ΔG d ) at all temperatures tested. Thermodynamic analysis of the results indicates that solvents with log λP ≤-2.3 have protective effects, whereas solvents with log λP ≥-1.8 are deleterious for penicillin acylase. |
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| Keywords: | Penicillin Acylase Streptomyces Lavendulae Organic Solvents Monophasic Systems Enzyme Stability |
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