Characteristics of sperm of captive seabass in relation to its fertilization potential |
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Authors: | C Fauvel § O Savoye C Dreanno † J Cosson ‡ M Suquet † |
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Institution: | Station Expérimentale d'Aquaculture, IFREMER, chemin de Maguelone, 34250 Palavas, France;Laboratoire de Physiologie des Poissons, IFREMER, 29280 Plouzané, France;URA 671 du CNRS, Universitéde Paris 6 Station Marine, 06230 Villefranche sur mer, France |
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Abstract: | Seabass Dicentrarchus labrax sperm concentration was high (up to 60 × 109 spz ml−1) but decreased significantly at the end of the reproductive season (mid-March) in monthly sampled fish. The spermiation period may be shortened by frequent stripping. Sperm can be prediluted up to 1: 128 in non-activating medium without loss of initial motility and motility duration. Immediately after activation by transfer to sea water, all the spermatozoa were motile for 10 s and then the number of motile cells decreased progressively but sharply to zero, so that the duration of sperm motility was very short (40 s). As a consequence, the fertility of seabass sperm decreased exponentially after 10 s following sperm activation and was zero by 1 min. The sperm requirements for optimal fertilization were c . 66 000 spermatozoa per egg. Scalingup of the experimental insemination procedure yielded better fertilization rates while conserving the individual differences due to the breeder pairs. |
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Keywords: | Dicentrarchus labrax seabass sperm concentration sperm motility artificial insemination gamete management |
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