普通小麦与簇毛麦原生质体的紫外线融合

从来源于普通小麦品种济南177（Triticum aestivum cv.Jinan 177）悬浮细胞系的原生质体与来源于簇毛麦（Haynaldia villosa）胚性愈伤组织的原生质体融合获得体细胞杂种。供体簇毛麦原生质体在融合之前用紫外线照射30s或1min，紫外线剂量为360Цw/cm^2。仅由紫外线照射30s的组合获得再生愈伤组织克隆。细胞学、生物化学及PCR分析结果证实了再生克隆的杂种性质。用线粒体基因特异的探针进行的RFLP分析的结果表明，杂种中含有融合双亲的线粒体并且发生了重组。由杂种愈伤组织再生得到白化苗。讨论了紫外线对融合产物的影响。

UV-fusion Between Protoplasts of Common Wheat and Haynaldia villosa

Intergeneric somatic hybrids were obtained by fusion between suspension cell-derived protoplasts of Triticum aestivum cv. Jinan 177 and protoplasts of Haynaldia villosa isolated from embryogeneric calli. Protoplasts of H. villosa were exposed to UV (360 μW/cm2) for 30 s and 1 min before fusion. Regenerated colonies were obtained only from the fusion combination in which the protoplasts of donor H. villosa were exposed to UV for 30 s. Results of cytological, biochemical and polymerase chain reaction (PCR) analysis of the 5S rDNA spacer sequence confirmed the hybrid nature of the regenerated colonies. Restriction fragment length polymorphism (RFLP) analysis with gene-specific mitochondrial probe showed that mitochondria from both parents existed and recombined in the hybrid clones. Albinos instead of normal green plants were regenerated from hybrid colonies. The effects of UV on fusion products were discussed.