Anomalies in 5'-end [32P] labelling of transfer RNA and partial sequence of a tRNAGlu from Scenedesmus obliquus |
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Authors: | J M McCoy D S Jones |
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Institution: | Department of Biochemistry, University of Liverpool, P.O. Box 147, Liverpool L69 3BX, U.K. |
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Abstract: | A chloroplast tRNAmMet species from is very poorly 5′-end ] labelled using γ-32P]ATP and T4 polynucleotide kinase. In sequencing the tRNA using standard 5′-labelled methods a very minor contaminating tRNA is preferentially labelled. The partial tRNA sequence determined by this method has an anticodon (CUC) for tRNAGlu. |
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