Tryptophan decarboxylase activity in transformed roots fromCatharanthus roseus and its relationship to tryptamine,ajmalicine, and catharanthine accumulation during the culture cycle |
| |
Authors: | Ignacio Islas Víctor M. Loyola-Vargas María de Lourdes Miranda-Ham |
| |
Affiliation: | (1) División de Biología Vegetal, Centro de Investigación Científica de Yucatán, 97310 Cordemex, Yucatán, Mexico |
| |
Abstract: | Summary Tryptophan decarboxylase (TDC), the enzyme that catalyzes the decarboxylation of tryptophan to trytamine, was studied in aCatharanthus roseus transformed root culture. Its activity was evaluated through the culture cycle (36 days), along with the variations in the tryptamine pool as well as the accumulation of alkaloids. Ajmalicine and catharanthine contents in the tissues increased coordinately with an increase in TDC-specific activity after 18 days of growth. No dramatic shifts were observed for the total alkaloid and tryptamine profiles. |
| |
Keywords: | Catharanthus roseus hairy root culture indole alkaloids tryptophan decarboxylase tryptamine pool |
本文献已被 SpringerLink 等数据库收录! |