A sensitive fluorimetric rate assay for biotinidase using a new derivative of biotin, biotinyl-6-aminoquinoline |
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| Authors: | H Wastell G Dale K Bartlett |
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| Affiliation: | 1. Department of Clinical Biochemistry, Newcastle General Hospital, Newcastle upon Tyne, United Kingdom;2. Department of Clinical Biochemistry and Metabolic Medicine, University of Newcastle upon Tyne, Newcastle upon Tyne, United Kingdom |
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| Abstract: | The previously reported method for the estimation of biotinidase (EC 3.5.1.12) is an endpoint colorimetric assay based on the hydrolysis of biotinyl-4-aminobenzoate, followed by diazotization, and is not suitable for our studies of biotinidase. A fluorimetric rate assay of biotinidase which uses a newly synthesized derivative biotinyl-6-aminoquinoline is described here. |
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| Keywords: | To whom correspondence should be addressed. |
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