Evaluation of Herpes Simplex Detection in Corneal Scrapings by Three Molecular Methods |
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Authors: | Amina Mostafa Abd El-Aal Maysaa El Sayed Eglal Mohammed Mohammed Ahmed Mona Fathy |
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Institution: | (1) Department of Clinical Pathology, Faculty of Medicine, Mansoura University, Mansoura, Egypt;(2) Department of Ophthalmology, Faculty of Medicine, Mansoura University, Mansoura, Egypt;(3) National Research Center, Cairo, Egypt |
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Abstract: | Herpes simplex virus (HSV)-1 keratitis (HSK) is a sight-threatening ocular infection with worldwide occurrence. A prompt laboratory
diagnosis is often very useful. The purpose of this study was to evaluate molecular methods as rapid diagnostic tools compared
with cell culture of HSK. Corneal scrapings from patients with clinically suspected HSK were tested by direct immunofluorescence
assay (IFA) for HSV-1 antigen and by polymerase chain reaction (PCR) for HSV-1 DNA, and an attempt for viral isolation was
performed on Vero cell line culture. Positive samples by cell culture were 20.8%, whereas PCR was positive in 29.2%, and IFA
was positive in 33.3%. IFA had better sensitivity (80%) and negative predictive value (81.8%) than PCR (70% and 76.9%, respectively);
however, PCR had better specificity (71.4%) and positive predictive value (63.6%). This indicates that a combination of cell
culture, IFA and PCR constitutes the best set of tools for diagnosis of clinically suspected cases of HSK. Documented infection
can be further assessed by cell-culture technique or PCR depending laboratory availability. |
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