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| 免疫抑制对EIAV弱毒疫苗免疫马体内疫苗弱毒株载量的影响 | |
| 引用本文： | 马建,姜成刚,林跃智,郭亮,郭巍,孔宪刚,沈荣显,邵一鸣,张晓燕,周建华.免疫抑制对EIAV弱毒疫苗免疫马体内疫苗弱毒株载量的影响[J].生物化学与生物物理进展,2008,35(11):1276-1281. |
| 作者姓名： | 马建 姜成刚 林跃智 郭亮 郭巍 孔宪刚 沈荣显 邵一鸣 张晓燕 周建华 |
| 作者单位： | 1. 中国农业科学院哈尔滨兽医研究所,兽医生物技术国家重点实验室,哈尔滨,150001;东北林业大学野生动物资源学院,哈尔滨,150040 2. 中国农业科学院哈尔滨兽医研究所,兽医生物技术国家重点实验室,哈尔滨,150001 3. 中国疾病预防控制中心性病艾滋病预防控制中心,北京,100050 |
| 基金项目： | 国家自然科学基金，黑龙江省发展高新技术产业专项，黑龙江省哈尔滨市科技攻关项目 |
| 摘 要： | 为了研究马传染性贫血弱毒疫苗EIAVFDDV在体内的生物学特性，特别是在免疫马血浆中低拷贝存在状态的形成原因，使用连续给予地塞米松的方法，对EIAVFDDV免疫马匹进行了免疫功能抑制．连续给药10天后，以植物血凝素（PHA）作为刺激原的淋巴细胞非特异性增殖实验表明，实验马匹的免疫系统功能受到了明显抑制．使用实时RT-PCR对免疫抑制前后实验马匹体内EIAVFDDV的载量进行了定量检测．结果显示，EIAVFDDV免疫马匹体内的病毒载量在2匹马中未见升高，1匹马体内略有增高，但仍处于EIAVFDDV在免疫马匹体内载量的正常范围．作为对照，强毒株隐性携带马的病毒载量提高了约25000倍，并出现了典型的临床症状．实验结果提示，致弱后病毒特有的生物学特性，而非免疫压力，是EIAV疫苗株在宿主血浆中低拷贝存在的主要原因．同时，在免疫系统功能受抑制状态下，疫苗株在免疫马体内仍然表现出了相对稳定的低拷贝复制状态，进一步显示了EIAVFDDV在应用中的安全性． |
| 关 键 词： | EIAVFDDV 减毒疫苗 免疫抑制 淋巴细胞增殖实验 病毒载量 |
| 收稿时间： | 2008/2/27 0:00:00 |
| 修稿时间： | 2008/4/16 0:00:00 |
The Plasma Load of Inoculated EIAV Vaccine Strain EIAVFDDV Was Not Boosted by Immune Suppression |
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| MA Jian,JIANG Cheng-Gang,LIN Yue-Zhi,GUO Liang,GUO Wei,KONG Xian-Gang,SHEN Rong-Xian,SHAO Yi-Ming,ZHANG Xiao-Yan and ZHOU Jian-Hua.The Plasma Load of Inoculated EIAV Vaccine Strain EIAVFDDV Was Not Boosted by Immune Suppression[J].Progress In Biochemistry and Biophysics,2008,35(11):1276-1281. | |
| Authors: | MA Jian JIANG Cheng-Gang LIN Yue-Zhi GUO Liang GUO Wei KONG Xian-Gang SHEN Rong-Xian SHAO Yi-Ming ZHANG Xiao-Yan and ZHOU Jian-Hua |
| Institution: | National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001, China;Wildlife Resources College, Northeast Forestry University, Harbin 150040, China;National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001, China;National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001, China;National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001, China;Wildlife Resources College, Northeast Forestry University, Harbin 150040, China;National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001, China;National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001, China;National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001, China;National Center for AIDS Control and Prevention, Beijing 100050, China;National Center for AIDS Control and Prevention, Beijing 100050, China;National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001, China |
| Abstract: | |
| Keywords: | EIAV FDDV |
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