Comparative study of the reactivation of oxygen evolution in chloroplasts inhibited by various treatments |
| |
Authors: | Yamashita, Takashi Tomita, Giiti |
| |
Affiliation: | Faculty of Agriculture, Kyushu University Fukuoka 812, Japan |
| |
Abstract: | Reactivation of photosynthetic oxygen-evolution was investigatedwith chloroplasts inhibited by 0.8 M Tris-, 0.8 M Tris-20% acetone-,0.8 M KCl-, 0.5 M NaClO4- or 1 mM NH2OH-washing, and with heat-treatedor aged chloroplasts. These chloroplasts restored oxygen evolvingactivity by two successive treatments; incubation of chloroplastswith reduced DPIP, then with Mn2$, Ca2$, dithiothreitol andbovine serum albumin under weak illumination (light-reactivation). Some factors required for light-reactivation could be omitteddepending on the inhibition treatment. For example, Mn2$, Ca2$and dithiothreitol were not necessary for (1 mM NH2OH-STN (pH7.0)-washed)-DPIP-treated chloroplasts, and dithiothreitol for(Tris-acetone (pH 8.4)-washed)-DPIP-treated chloroplasts. Uncouplers, such as atebrin, CCCP, DCCD and NH4Cl, inhibitedthe lightreactivation. The Mn and Ca contents of the chloroplasts were determined withinhibited and DPIP-treated chloroplasts. The Mn content of thechloroplasts tended to decrease with increasing pH of the washingmedium for inhibition. The Ca content decreased when chloroplastswere washed with 0.8 M KCl. (Received November 22, 1974; ) |
| |
Keywords: | |
本文献已被 Oxford 等数据库收录! |
|