Molecular characterization of the soybean L-asparaginase gene induced by low temperature stress |
| |
Authors: | Cho Chang-Woo Lee Hye-Jeong Chung Eunsook Kim Kyoung Mi Heo Jee Eun Kim Jung-In Chung Jongil Ma Youzhi Fukui Kiichi Lee Dae-Won Kim Doh-Hoon Chung Young-Soo Lee Jai-Heon |
| |
Affiliation: | Department of Genetic Engineering, Dong-A University, Busan 604-714, Korea. |
| |
Abstract: | L-asparaginase (EC 3.5.1.1) catalyzes the hydrolysis of the amide group of L-asparagine, releasing aspartate and NH4+. We isolated a low temperature-inducible cDNA sequence encoding L-asparaginase from soybean leaves. The full-length L-asparaginase cDNA, designated GmASP1, contains an open reading frame of 1,258 bp coding for a protein of 326 amino acids. Genomic DNA blotting and fluorescence in situ hybridization showed that the soybean genome has two copies of GmASP1. GmASP1 mRNA was induced by low temperature, ABA and NaCl, but not by heat shock or drought stress. E. coli cells expressing recombinant GmASP1 had 3-fold increased L-asparaginase activity. A possible function of L-asparaginase in the early response to low temperature stress is discussed. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|