In vitro propagation of a semi-dwarfing cherry rootstock |
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Authors: | Muna AL-Sabbagh Ahmad Abdul-Kader Mahmoud Khoder Abdul-Rahman Kalhout |
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Institution: | (1) Ministry of Agriculture (SMAAR), Directorate of Agricultural Scientific Research, (DASR)-Douma, PO Box 113, Damascus, Syria;(2) Agricultural Engineering Faculty, Horticulture Department, Alleppo University, Aleppo, Syria |
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Abstract: | A successful in vitro propagation system for the semi-dwarfing cherry rootstock Maxma-14 (Prunus avium L.) has been developed. Shoot tips and axillary buds were successfully established in vitro. Multiplication rate of about 6 was achieved over a 4-week period using Murashige and Skoog medium with 4.44 μM benzyladenine
and 0.49 μM indole-3-butyric acid (IBA). Rooting occurred within 4 weeks on liquid and agar-gelled media containing 0.49 μM
NAA or 0.49, 2.45 μM IBA. On liquid media, a maximum rooting efficiency of up to 100% was obtained. However, high concentrations
of auxins delayed the time of root initiation for 3–5 days. Acclimatization was affected directly by rooting conditions. Survival
was best when plantlets were transferred to pots after a short period of root emergence on rooting media. Multiplication medium
was also important for successful acclimatization. Shoots transferred to rooting media from that with kinetin resulted in
better acclimatization and survival than that derived from media with benzyladenine. Further, plantlets rooted on liquid media
had better survival than that rooted on agar-gelled media.
This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | growth regulators Maxma-14 micropropagation Prunus avium L tissue culture |
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