Complete nucleotide sequence of a novel porcine circovirus-like agent and its infectivity in vitro |
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Authors: | LiBin Wen KongWang He HanChun Yang YanXiu Ni XueHan Zhang RongLi Guo QunXin Pan |
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Institution: | (1) Key Laboratory of Animal and Poultry Diseases Diagnostic of the Ministry of Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China;(2) Key Laboratory of Preventive Veterinary Medicine of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, 100094, China |
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Abstract: | A novel agent (hence termed as P2) was isolated from pig sera in China, which contained covalently bound circular genomic
DNAs of 993 nucleotides. Sequence analyses indicated that the agent was closely related to the porcine circovirus (PCV). The
molecular clone of P2 was constructed subsequently and used for the following studies. Intracytoplasmic inclusions and intranuclear
inclusions were only found in PK-15 cells transfected with the tandem dimer of P2 molecular DNA clone. Intracytoplasmic inclusions
were round or irregular in shape and 0.1–0.4 μm in diameter, and intranuclear inclusions were electronically denser than intracytoplasmic
inclusions and had two general shapes: round/small (0.1 μm in diameter) and hexagonal/large (0.5–1.4 μm in diameter). The
inclusions were not membranously bound. The cells transfected with the tandem dimer of P2 molecular DNA clone were tested
positive for P2 DNA at passages 5. The P2 antigen could be detected in both transfected and passaged PK-15 cells. This is
the first report regarding the complete nucleotide sequence of a small DNA genome in a circovirus-like infectious agent in vitro.
Supported by the State Key Basic Research Project (973 project) of China (Grant No. 2007CB116308), Planned Projects for Postdoctoral
Research Funds of Jiangsu Province, China (Grant No. 5910602) and Postdoctoral Funds of Jiangsu Academy of Agricultural Sciences,
China (Grant No. 6510501) |
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Keywords: | P2 porcine circovirus molecular clone PK-15 cells |
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