Mammalian cell cultivation on serum-coated microcarriers |
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| Authors: | Byung-Soo Kim Youn Hee Choi Cha Yong Choi Byung Gee Kim |
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| Institution: | (1) Department of Chemical Technology, College of Engineering, 151-742 Seoul, Korea;(2) Interdisciplinary Program for Biochemical Engineering and Technology, College of Engineering, 151-742 Seoul, Korea;(3) Institute for Molecular Biology and Genetics, Seoul National University, 151-742 Seoul, Korea |
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| Abstract: | Summary The cell culture on serum-coated microcarriers yielded higher efficiency of cell attachment to microcarriers and more favorable
initial cell distribution on microcarriers than on the conventional microcarriers. By employing serum-coated microcarriers,
the maximum cell density was increased by 46% in low serum medium and by 30% in 10% (v/v) serum-supplemented medium. Serum
coating of microcarriers could provide cell attachment factors and may replace costly attachment factors supplemented in low
serum medium and serum-free medium. |
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