Analytical assays of human HSP27 and thermal-stress survival of Escherichia coli cells that overexpress it |
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Authors: | Takeuchi Satoru |
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Institution: | Department of Protein Research, ProstaColon, 85 NE, Takamatsu, Kahoku, Ishikawa 929-1215, Japan. takeuchi@coffee.ocn.ne.jp |
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Abstract: | HSP27 is a small heat-shock protein (sHSP). Such proteins are produced in all organisms. These small HSPs exhibit chaperone-like activity that can bind to unfolded polypeptides and prevent uncontrolled protein aggregation in vitro. Cellular anti-apoptosis function and enhanced cell survival are correlated with increased expression of HSPs. This study presents a thermal-stress survival model for cells using the Escherichia coli expression system for which human HSP27, a recombinant protein, is inducible. Results show that E. coli cells overexpressing human HSP27 have enhanced tolerance to 50 degrees C thermal stress. |
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Keywords: | HSP27 Chaperone-like activity Hydropathy Thermotolerance |
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