Site-directed antisera to the chromophore binding site of phytochrome: characterization and cross-reactivity |
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| Authors: | F M Mercurio R A Houghten J C Lagarias |
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| Affiliation: | 1. Faculty of Biology, M.V. Lomonosov Moscow State University, Moscow, Russia;4. Siberian State Medical University, Tomsk, Russia;5. National Research Tomsk State University, Tomsk, Russia;1. Traffic Injury Research Foundation, Ottawa, Canada;2. Washington University at St. Louis, MO, United States;1. Department of Psychiatry and Human Behavior, Brown Medical School, Rhode Island Hospital, Providence, United States;2. Department of Psychiatry, Rhode Island Hospital, Providence, United States;1. Department of Occupational Therapy and Graduate Institute of Behavioral Sciences, Chang Gung University, Taoyuan, Taiwan;2. Healthy Aging Research Center, Chang Gung University, Taoyuan, Taiwan;3. Department of Psychiatry, Chang Gung Memorial Hospital, Linkou, Taiwan;4. Laboratory of Brain Imaging and Neural Dynamics (BIND Lab), Chang Gung University, Taoyuan, Taiwan;5. College of Medicine, Chang Gung University, Taoyuan, Taiwan |
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| Abstract: | ![]()
The preparation and characterization of antisera to a synthetic undecapeptide which encompasses the chromophore binding site on oat phytochrome are described. By direct enzyme-linked immunosorbent assay, this antiserum recognizes bilin-linked peptides in proteolytic digestion mixtures of phytochrome from several plant species including the monocot species, oat and maize, and the dicot species, pea. Competitive enzyme-linked immunosorbent assays have been used to establish the specificity of the site-directed antisera for those peptides homologous with the synthetic undecapeptide which are present in complex mixtures. These results show that the local environment of the phytochromobilin prosthetic group is structurally conserved. |
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