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Heavy-metal (Zn,Cd) tolerance in selected clones of duck weed (Lemna minor)
Authors:R F M Van Steveninck  M E Van Steveninck  D R Fernando
Institution:(1) School of Agriculture, La Trobe University, 3083 Bundoora, Australia;(2) Department of Botany, La Trobe University, 3083 Bundoora, Australia
Abstract:Cryo-microprobe analysis of quench-frozen fronds of a Zn-tolerant clone of Lemna minor exposed to a high level of Zn (300 μM) showed the presence of cellular deposits consisting of Zn, Mg, K and P or Zn, K and P (Zn phytate). The same Zn-tolerant clone of Lemna minor, when exposed to a high level of Cd (30 μM), showed the presence of globular deposits consisting of Cd, K and P in mature fronds, but the immature cells of the enclosed daughter fronds contained relatively large deposits with Cd and S as the main components (Cd-phytochelatin?). Selection for Zn tolerance in a population of Lemna minor was easily achieved but selection for Cd tolerance has so far not been successful. The Zn-tolerant clone also tolerates high levels of phosphate.
Keywords:cadmium  clones  heavy metal tolerance  Lemna minor  oxalate  phosphate  phytic acid  phytochelatin  sulphur  X-ray microanalysis  zinc
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