Recovery from radiation-induced decrease in cell membrane charge by added adenosine triphosphate and its modification by colchicine or cytochalasin B. |
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Authors: | C Sato K Kojima K Mishizawa |
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Affiliation: | 1. Department of Experimental Radiology, Aichi Cancer Center Institute, Chikusa-ku, Nagoya 464, Japan;7. Laboratory of tumor Pathology, Nara Medical College, Nara, Japan |
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Abstract: | Cell electrophoretic mobility of rat erythrocyte decreased with time after 3000 R X-irradiation without spontaneous recovery. On addition of 10?4M ATP to the irradiated cells, recovery was observed within 10 minutes. Washing out of ATP and subsequent incubation for 1 hr resulted in the return of mobility to the low level. Preincubation with 0.1 μg/ml colchicine for 15 minutes or 1 μg/ml cytochalasin B for 30 min completely blocked the reversible effect of ATP on electrophoretic mobility. These results suggest the existence of tubulin-like polymerizing protein in the cytoplasmic membrane and changes in its conformation induced both by X-irradiation and by added ATP. |
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