Tissue-Specific Induction of Hsp90 mRNA and Plasma Cortisol Response in Chinook Salmon following Heat Shock, Seawater Challenge, and Handling Challenge |
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| Authors: | Aldo N Palmisano James R Winton Walton W Dickhoff |
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| Institution: | (1) Western Fisheries Research Center, Biological Resources Division, U.S. Geological Survey, 6505 NE 65th Street, Seattle, WA 98115, U.S.A., US;(2) University of Washington, School of Fisheries, Seattle, WA 98195, U.S.A., US;(3) National Marine Fisheries Service, Seattle, WA 98112, U.S.A., US |
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| Abstract: | In studying the whole-body response of chinook salmon (Oncorhynchus tshawytscha) to various stressors, we found that 5-hour exposure to elevated temperature (mean 21.6°C; + 10.6°C over ambient) induced
a marked increase in Hsp90 messenger RNA accumulation in heart, brain, gill, muscle, liver, kidney, and tail fin tissues.
The most vital tissues (heart, brain, gill, and muscle) showed the greatest Hsp90-mRNA response, with heart tissue increasing
approximately 35-fold. Heat shock induced no increase in plasma cortisol. In contrast, a standard handling challenge induced
high plasma cortisol levels, but no elevation in Hsp90 mRNA in any tissue, clearly separating the physiological and cellular
stress responses. We saw no increase either in tissue Hsp90 mRNA levels or in plasma cortisol concentrations after exposing
the fish to seawater overnight.
Received October 1, 1999; accepted January 21, 2000 |
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| Keywords: | : Chinook salmon Oncorhynchus tshawytscha heat shock Hsp90 mRNA cortisol handling stress |
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