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黑曲霉木聚糖酶的纯化与性质
引用本文:陈红歌 朱静 梁改芹 严自正 贾新成 张树政. 黑曲霉木聚糖酶的纯化与性质[J]. 菌物学报, 2000, 19(1)
作者姓名:陈红歌 朱静 梁改芹 严自正 贾新成 张树政
作者单位:中国科学院微生物研究所
基金项目:国家自然科学基金!39600029,国家“九五”科技攻关项目!No.96-C03-02-03
摘    要:由凝胶电泳酶谱检测到黑曲霉149发酵液中存在两型木聚糖酶,依次为X-Ⅰ和X-Ⅱ.通过硫酸铵分级沉淀及DEAE-SephadexA50柱层析分别将X-Ⅰ、X-Ⅱ纯化到凝胶电泳均一。由SDS一凝胶电泳和浓度梯度凝胶电泳测得X-Ⅰ和X-Ⅱ的分子量分别为37kDa,76kDa,24kDa和23kDa,X-Ⅰ具有亚基。二者的含糖量分别为276%和7.3%。X-Ⅰ和X-Ⅱ最适反应温度分别为50℃和55℃,pH为46和5.2。在pH4.6~9.2和pH4.0~10.0之间X-Ⅰ、X-Ⅱ活力稳定。50℃保温24h,X-Ⅰ活力仍为100%,而X-Ⅱ的活力已降为2.8%。HgCl2和AgNO3显著抑制X-Ⅰ、X-Ⅱ的活力。X-Ⅰ与X-Ⅱ水解不同来源的木聚糖,其产物有所不同。

关 键 词:黑曲霉  木聚糖酶  纯化  性质

PURIFICATION AND SOME PROPERTIES OF XYLANASEFROM ASPERGILLUS NIGER
CHEN Hong-Ge, ZHU Jing, LIANG Gai-QinYAN Zi-Zheng,JIA Xin-Cheng, ZHANG Shu-Zheng. PURIFICATION AND SOME PROPERTIES OF XYLANASEFROM ASPERGILLUS NIGER[J]. Mycosystema, 2000, 19(1)
Authors:CHEN Hong-Ge   ZHU Jing   LIANG Gai-QinYAN Zi-Zheng  JIA Xin-Cheng   ZHANG Shu-Zheng
Abstract:Zymograms of the cultrual supernatant of Aspergillus niger 149 showed xylanase's two bandstentatively named X-I and X-II. X-I and X-II were purified to PAGE homogenity by (NH4)2So4 fractionationand DEAE-Sephadex A50 column chlomatography. The molecular mass of X-I and X-II eshmated by SDS-PAGEand concentrahon gradient PAGE were 37kDa,76kDa,24kDa, and 23kDa. respechvely. X-I and X-II contained27.6% and 7.3% carbohydrate residues, respechvely. The optimal reachon conditions for X-I and X-II were at50℃ pH4.6 and 55℃ PHS.2. X-I and X-II retained 100% and 2.8% respechvely, of the original activity afterheating at 50℃ for 24h. The achvihes of X-I and X-II were considerably inhibited by HgCl2 and AgNO3. Thehydrolysis products from different xylan with X-I and X-II were different.
Keywords:mpergillus niger  Xylanase   Purification  Propertis.
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