Enhancement of the releases of GABA and glycine during ischemia in rat spinal dorsal horn |
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Authors: | Kawasaki Yasuhiko Fujita Tsugumi Kumamoto Eiichi |
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Institution: | Department of Physiology, Saga Medical School, Saga 849-8501, Japan. |
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Abstract: | The present study examined a change in spontaneous inhibitory postsynaptic currents (sIPSCs) following ischemia in substantia gelatinosa (SG) neurons of adult rat spinal cord slices by using the whole-cell patch-clamp technique. At about 10 min after superfusion of an oxygen- and glucose-free medium, sIPSCs were remarkably increased in amplitude and frequency when compared with those in the control. In a phase of the increase in sIPSC activities, GABAergic and glycinergic sIPSCs, which were observed in the presence of strychnine and bicuculline, respectively, with TTX, were increased greatly in frequency with a minimal change in their amplitudes. It is concluded that the in vitro ischemia increases the spontaneous quantal releases of GABA and glycine to SG neurons from nerve terminals; a part of this enhancement is possibly due to an increase in spontaneous activities of inhibitory interneurons. GABA released thus might serve to inhibit the release of l-glutamate from nerve terminals. |
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Keywords: | Ischemia GABA Glycine Substantia gelatinosa Spinal dorsal horn Slice preparation Patch-clamp |
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