Functional and morphological evidence for the existence of neurites from abdominal ganglion bag cell neurons in the head-ring ganglia of Aplysia |
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Authors: | Stephen B Shope Duane McPherson Michael K Rock James E Blankenship |
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Institution: | (1) Marine Biomedical Institute, University of Texas Medical Branch, 200 University Boulevard, 77550 Galveston, TX, USA;(2) Present address: Department of Neurology (D4-5), University of Miami School of Medicine, Box 016960, 33101 Miami, FL, USA;(3) Present address: Department of Biology, Marquette University, 53233 Milwaukee, WI, USA |
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Abstract: | Summary Three lines of evidence are presented indicating that axons of the Aplysia neuroendocrine bag cells extend into the head-ring ganglia of the CNS. When the abdominal ganglion was bisected longitudinally, separating the two bag cell clusters, an afterdischarge induced in one cluster generated an afterdischarge in the other via activity through the head-ring ganglia to which each half abdominal ganglion was attached by connective nerves. This suggests that some axons of bag cells in each cluster communicate through the head-ring ganglia. Retrograde labelling of bag cells occurred when rhodamine-onjugated latex microspheres were injected into the cerebral or either pleural ganglion, a direct demonstration that bag cell axons extend into these ganglia. Finally, cell LP1 in the left pleural ganglion was inhibited during a bag cell afterdischarge, an action mimicked by application of alpha-bag cell peptide ( BCP). Since BCP can act only close to its site of release due to susceptibility to peptidase activity, it is likely that LP1 inhibition is dependent on the local release of BCP from bag cell neurites in the pleural ganglion. These results open new possibilities for how bag cell afterdischarges may be initiated and broaden the distribution of their effects.Abbreviations
ASW
artificial sea water; -BCP -bag cell peptide
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ELH
egg-laying-hormone
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IR
immunorective
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PB
phosphate buffer
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PVC
pleurovisceral connective |
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Keywords: | Aplysia Bag cells Retrograde labelling Rhodamine conjugated latex microspheres Egglaying behavior |
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