Isolation and random amplified polymorphic DNA analysis of genomic DNA from soybean embryos |
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| Authors: | Paul N. McMahon Marla S. McIntosh |
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| Affiliation: | (1) Department of Agronomy, University of Maryland, College Park, USA;(2) Present address: H.J. Patterson Hall, University of Maryland, 20742 College Park, Maryland, USA |
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| Abstract: | Summary An isolation procedure was developed for the extraction of genomic DNA and Random Amplified Polymorphic DNA (RAPD) analysis using individual soybean embryos. This procedure can be used to quickly and efficiently isolate DNA from a large number of individuals. DNA isolations were analyzed for total yield, integrity, and usefulness as a template in RAPD analysis. |
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