Degeneration of solventogenic Clostridium strains monitored by Fourier transform infrared spectroscopy of bacterial cells |
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Authors: | KC Schuster R Goodacre JR Gapes M Young |
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Institution: | (1) Institute of Chemical Engineering, Fuel and Environmental Technology, Vienna University of Technology, Vienna A1060, Austria, AT;(2) Institute of Biological Sciences, Cledwyn Building, University of Wales Aberystwyth, Aberystwyth, Ceredigion SY23 3DD, UK, GB |
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Abstract: | Strain degeneration in solventogenic clostridia is a known problem in the technical acetone–butanol fermentation bioprocess,
especially in the continuous process mode. Clostridial strain degeneration was studied by Fourier transform infrared (FT-IR)
spectroscopy of the bacterial cells. Degenerative variant formation in two strains, Clostridium beijerinckii NCIMB 8052 and Clostridium species AA332, was detected spectroscopically. Colonies on solid media were sampled, or assayed directly in situ by IR microscopy. It has previously been shown that the distinctive acidogenic and solventogenic physiological phases of
Clostridium acetobutylicum in liquid medium can be discriminated by FT-IR spectroscopy. This was confirmed here for C. beijerinckii NCIMB 8052. The proportion of degenerate cells in a mixed population in liquid medium could be quantified, as the spectral
features change in different ways during the normal growth cycle of wild type organisms and degenerate variants in batch culture.
This opens a new perspective for physiology-based process monitoring and control, especially of the continuous acetone–butanol
fermentation.
Journal of Industrial Microbiology & Biotechnology (2001) 27, 314–321.
Received 06 October 2000/ Accepted in revised form 20 April 2001 |
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Keywords: | : Acetone-butanol fermentation Clostridium solventogenic fermentation strain degeneration FT-IR spectroscopy fermentation process monitoring |
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