改性甲壳素固定化胰蛋白酶直接纯化牛肺Kunitz抑制剂的研究

通过对天然甲壳素 (或壳聚糖 )进行化学改性并修饰作为载体 ,再经氯代环氧丙烷活化偶联 ,制成固定化胰蛋白酶亲和吸附剂 (蛋白酶偶联率为 6 2 1% ,酶活性回收率为 5 7 8% ) ,直接亲和层析牛肺提取液中Kunitz抑制剂。纯化的产品每毫克蛋白酶抑制剂活力相当于 5 82 0BAEETTU/mg蛋白质 ,纯化率为 40 7。提高了Kunitz抑制剂的稳定性能和回收率 ,简化了工业化生产程序 ,具开发前景

STUDY ON DIRECT PURIFICATION TO KUNITZ INHIBITOR FROM BOVINE LUNGS WITH TRYPSIN FIXED BY MODIFIED CHITOSAN

After chemi-modified,the nature chitin was used as carrier.When this vector was activated with epichlorohydrin and coupled with bovine trypsin,a kind of affinity absorbent,immobilized trypsin,was made(the rate of protein coupling was 62.1% and the rate of trypsin activity recovery was 57.8%).Kunitz inhibitor from bovine lungs could be directly extracted through the affinity chromatography.The activity of these products was equal to 5820 BAEE TTU/mg and the rate of purification was 407.This method made the Kunitz inhibitor stable,the rate of recovery improved and the industrial working process simple.