Cloning, quantification and characterization of a minisatellite DNA sequence from common bean Phaseolus vulgaris L. |
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Authors: | I Métais C Aubry B Hamon D Peltier R Jalouzot |
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Institution: | (1) Laboratoire de Génétique, UFR Sciences, 2BD Lavoisier, F-49045 Angers Cedex, France Fax: +33-2-41735352, FR |
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Abstract: | We describe the cloning and the characterization of a 130-bp DNA fragment, called OPG9-130, amplified from bean (Phaseolus vulgaris L.) genomic DNA. This fragment corresponds to a minisatellite DNA sequence containing seven repeats of 15 bp which differ
slightly from each other in their sequence. Southern analysis showed that the core sequence of 15 bp is repeated in clusters
dispersed throughout the genome. The use of this fragment as a probe allowed us to identify common bean lines by their DNA
fingerprints. We suggest that OPG9-130 will be useful for line identification as well as for the analysis of genetic relatedness
between bean species and lines.
Received: 14 February 1998 / Accepted: 10 February 1998 |
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Keywords: | Minisatellite DNA fingerprinting DNA polymorphism Phaseolus |
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