Two-Dimensional Electrophoretic Analysis of Salicylic Acid-Induced Changes in Polypeptide Pattern of Barley Leaves |
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| Authors: | MV Metodiev MI Kicheva ZhG Stoinova LP Popova |
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| Institution: | (1)  M. Popov Institute of Plant Physiology, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bl. 21, BG-1113 Sofia, Bulgaria |
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| Abstract: | The salicylic acid-induced changes in the polypeptide patterns of barley (Hordeum vulgare L.) leaves have been analysed using two-dimensional gel electrophoresis. An optimized 2-D PAGE protocol was used and gave reproducible 2-D gels from leaf crude protein extracts with a high number of detected polypeptides. When applied for 24 h SA affected the expression of a number of soluble proteins. Most of them appeared to be down-regulated. Although no abundant expression of specific proteins was observed, we detected three polypeptides that were present only in SA-treated leaves. |
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| Keywords: | 2-D PAGE Hordeum vulgare leaf proteins |
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