Optimizing the fermentation conditions and enhanced production of keratinase from Bacillus cereus isolated from halophilic environment |
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Authors: | S. Arokiyaraj R. Varghese B. Ali Ahmed V. Duraipandiyan N.A. Al-Dhabi |
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Affiliation: | 1. Department of Food Science and Technology, Sejong University, Republic of Korea;2. Department of Biotechnology, PRIST University, Thanjavur, Tamil Nadu 613401, India;3. Faculty of Applied Sciences, Ton Duc Thang University, Go Chi Minh City, Viet Nam;4. Department of Botany and Microbiology, College of Science, King Saud University, P. O. Box 2455, Riyadh 11451, Saudi Arabia |
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Abstract: | Keratinase degrading Bacillus cereus was isolated from the halophilic environment in Tamilnadu, India and keratinase production was optimized using wheat bran substrate. Of the screened bacterial isolates, four were found to have the ability to produce keratinolytic enzyme. The process parameters were optimized using one-variable-at-a-time approach and response surface methodology. Supplementation of 1% lactose supported more keratinase production (120?U/g). Among the selected nitrogen sources, addition of casein significantly enhanced maximum keratinase production (132.5?U/g). Among the ions, manganese chloride significantly enhanced keratinsase production (102.6?U/g), however addition of zinc sulphate and copper sulphate decreased keratinase production. The maximum keratinase production was obtained in the wheat bran medium containing 1% lactose, 0.5% manganese with 80% moisture (292?U/g). Statistics based contour plots were generated to explore the variations in the response surface and to find the relationship between the keratinase yield and the bioprocess conditions. |
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Keywords: | Kerainase Response surface methodology Halophilic |
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