Effects of ginsenosides on carbachol-stimulated formation of inositol phosphates in rat cortical cell cultures |
| |
Authors: | Lee Jun-Ho Choi Seok Kim Jong-Hoon Kim Jong-Keun Kim Jae-il Nah Seung-Yeol |
| |
Affiliation: | (1) Department of Physiology, College of Veterinary Medicine, Konkuk University, Seoul, 143–701, Korea;(2) Department of Pharmacology, Chonnam National University Medical School, Kwangju, Korea;(3) Department of Life Science, Kwnangju Institute of Science and Technology, Kwangju, 500–712, Korea |
| |
Abstract: | We examined the effect of ginseng total saponins (GTS) on phosphoinositide metabolism stimulated by activation of muscarinic receptor using rat cortical cultures. Carbachol stimulated formation of [3H]inositol phosphates ([3H]InsPs) by 3.3-fold over basal level in [3H]inositol-prelabeled cells. Pretreatment of GTS inhibited formation of [3H]InsPs evoked by carbachol by 70%–90%. Addition of GTS alone had no effect on the basal formation of [3H]InsPs. The inhibitory effect of the GTS on carbachol-stimulated formation of [3H]InsPs was dose- and time-dependent. IC50 was 6.0 ± 2.8 g/ml. We also examined the effect of GTS on [3H]InsP1, [3H]InsP2, or [3H]InsP3 formation evoked by carbachol. Although GTS had no effect on the basal [3H]InsP1, [3H]InsP2, or [3H]InsP3 formation, pretreatment of GTS inhibited [3H]InsP1, [3H]InsP2, or [3H]InsP3 formation evoked by carbachol, respectively. Addition of individual ginsenosides such as ginsenoside Rb1, Rc, Rd, Re, or Rg2 had no effect on the basal formation of [3H]InsPs, whereas pretreatment of ginsenoside Rb2, Rc, Rd, Re, Rf, Rg1 or Rg2 inhibited formation of [3H]InsPs evoked by carbachol by 79%–89%. The results suggest that the inhibitory effect of GTS and its individual ginsenosides on carbachol-stimulated formation of [3H]InsPs in cortical neurons could be one pharmacological action of Panax ginseng. |
| |
Keywords: | Ginsenosides carbachol inositol phosphates cortical neurons |
本文献已被 PubMed SpringerLink 等数据库收录! |
|