The vacuolar membrane of plant cells: a newcomer in the field of biological membranes |
| |
| Authors: | H Barbier-Brygoo J P Renaudin J Guern |
| |
| Institution: | 1. Department of Systems Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea;2. Division of Biological Science and Technology, Yonsei University, Wonju, 220-710, Republic of Korea;3. Molecular Breeding Division, Natural Institute of Agricultural Sciences, Rural Development Administration, Jeonju 55365, Republic of Korea;1. Department of Chemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand;2. Department of Microbiology, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand;3. Natural Product Research Center of Excellence, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand;4. Environmental Futures Research Institute, Griffith University, Gold Coast, Queensland 4222, Australia;5. Eskitis Institute, Griffith University, Brisbane, Queensland 4111, Australia;1. National Engineering Laboratory for Rice and By-product Deep Processing, Central South University of Forestry and Technology, Changsha, 410004, Hunan Province, PR China;2. Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, Hunan University, Changsha, 410082, PR China;3. NJU-NJFU Joint Institute for Plant Molecular Biology, School of Life Sciences, Nanjing University, Nanjing, 210093, PR China;4. Department of Plant and Microbial Biology, University of California, Berkeley, CA, 94720, USA;1. Cell Biology and Biophysics Unit, National Institute of Neurological Disorders and Stroke, Bethesda, MD 20892, USA;2. Biophysics Center, National Heart, Lung and Blood Institute, MD 20892, USA |
| |
| Abstract: | The vacuole of plant cells is a large compartment whose functions (storage, regulation of cytoplasmic homeostasis) have been studied mainly using indirect methods. The recent development of procedures to isolate this organelle enables direct investigation of its properties and of those of its surrounding membrane, the tonoplast. Several problems are encountered when studying the tonoplast: the small quantities of membrane material recovered, the contamination by other membranes, and the lack of an unambiguous marker. This accounts for the scarcity of analytical data concerning this structure. The investigation of transport systems at the tonoplast was stimulated by the existence of a high pH gradient across this membrane and the accumulation of various solutes inside the vacuole (sucrose, organic acids, etc.). Up to now, the most studied system is the proton-pumping ATPase. Its main characteristics are presented, together with a few data on other transport systems. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
