Cloning and Characterization of New Repetitive Sequences in Field Bean (Vicia fabaL.) |
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Authors: | NOUZOVA MARCELA; KUBALAKOVA MARIE; DOLEZELOVA MARIE; KOBLIZKOVA ANDREA; NEUMANN PAVEL; DOLEZEL JAROSLAV; MACAS JIRI |
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Institution: | Institute of Plant Molecular Biology, Brani ovská 31, eské Bud jovice, CZ-370 05, Czech Republic Institute of Experimental Botany, Sokolovská 6, CZ-77200, Olomouc, Czech Republic |
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Abstract: | Five new repetitive sequences have been isolated from theViciafabagenome, by cloning bands visible on agarose gel electrophoresisafter digestion of genomic DNA with various restriction enzymes.The sequences were 109 to 584 bp long, their abundance rangingfrom 5x104to 5x105copies per haploid genome. Southern blot andinsituhybridization revealed that four of five newly isolatedrepeats were dispersed in theV. fabagenome. One of the repeats(TIII15) showed tandem organization with several major hybridizationspots on mitotic chromosomesin situ.These sites were distributedin euchromatic as well as in heterochromatic chromosomal regions,and in several loci they were simultaneously localized withpreviously describedFokI repeated elements. The sequence ofTIII15 comprises four 2627 bp subrepeats, but sharesno homology toFokI elements which have similar sequence organization.All newly described sequences were highly specific forV. faba,withlittle or no hybridization to DNA of otherViciaspecies, andno hybridization to DNA of other legumes tested.Copyright 1999Annals of Botany Company Vicia faba, field bean, repeated DNA sequences, FISH, PRINS, genome organization, copy number. |
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