IDENTIFICATION OF A PROTEIN FRACTION IN THE OCCIPITAL CORTEX OF THE MONKEY RAPIDLY LABELLED DURING THE EXPOSURE OF THE ANIMAL TO RHYTHMICALLY FLICKERING LIGHT |
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Authors: | U. B. Singh G. P. Talwar |
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Affiliation: | Department of Biochemistry, All-India Institute of Medical Sciences, New Delhi-16, India |
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Abstract: | Monkeys exposed to a rhythmically flickering light (flicker frequency 7/sec, intensity 1614 lumens/m2) show a higher incorporation of intracisternally administered l -(U-3H)-lysine into proteins of the visual cortex as compared to monkeys kept in darkness. An increase in specific radioactivity is noticed in both the soluble and particulate (including membrane linked) proteins. The 105,000 g supernatant proteins from the visual cortex have been fractionated on DEAE-cellulose columns followed by resolution of each fraction on polyacrylamide gels. The results suggest that there is a group of acidic low molecular weight proteins whose synthesis is significantly stimulated during the exposure of the animal to flickering light. The fractions give immunological cross-reaction with anti S-100 Serum. |
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